Discussions

Scientists at Creative Biolabs have used almost all available approaches for the delivery of reprogramming factors. RNA reprogramming can be achieved through the transfection/transduction of synthetic mRNAs, microRNAs (miRNAs), RNA viruses, or synthetic RNA replicons. Direct delivery of reprogramming RNA (synthetic modified mRNA) can avoid integration of the transgene sequences. To facilitate the transition of iPS cells to clinical practice, a variety of technologies have been developed for transgene-free pluripotency reprogramming at Creative Biolabs, including reprogramming factor delivery by mRNA.