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The process of precipitating a protein antigen out of solution with an antibody that binds to that specific protein precisely is known as immunoprecipitation (IP). A specific protein can be separated and concentrated with this method from a sample that contains thousands of other proteins.

Specific Protein IP
An antibody is used in individual protein IP to separate a particular target protein from cell lysate. Protein A/G-coupled agarose or magnetic beads are used to extract the antibody-antigen complex from the sample after the antibody binds to the protein. The protein is eluted after washing the beads. Several molecular methods, including Western blot (WB) and Enzyme-linked Immunosorbent Assay (ELISA), are used to verify the purified antigen produced by IP.