Discussions
Single Cell Sorting
Sorting cells by type from sample materials is accomplished using single-cell sorting. The main criteria used to isolate them include variations in morphology (or size), expression of surface proteins, and other characteristics. The usual process involves obtaining viable, undamaged cells through enzymatic or mechanical dissociation, followed by the isolation of individual cells from the dissociated suspension. The two groups that comprise the present cell isolation technologies are based on the diversity of concepts.
We can see that our technologies consist of, but are not restricted to:
Fluorescence-activated cell sorting (FACS): many parameters, excellent selectivity, and throughput
Magnetic-activated cell sorting (MACS): a cost-effective method with excellent throughput and specificity
Whole, preserved, and living tissue are dissected using laser capture microdissection (LCM).