Discussions

Our MAPPS assay works efficiently with a turnaround time of 3-4 weeks. Firstly, antigen-presenting cells can figure out the protein and process/present peptide fragments existed in MHC molecules on the cell surface. Then, cells will be lyzed and HLA-peptide molecules will be recovered in an immune affinity step. Finally, peptides will be separated from the HLA molecules and be analyzed by LC-MS/MS. In this process, we are capable of identifying sequences after subjecting to rigorous analysis to identify true positive peptides with high confidence.