Discussions

Reprogramming by Virus
Retro-and lentivirus: Retro- and lentiviral delivery vectors have been used to generate iPSCs almost every lab, because this viral transduction is easily implemented in most biomedical research labs. Using this delivery system, the reprogramming efficiency was 0.01–0.02% in human cells with iPSC colonies occurring between 25 and 30 days post-infection. As retroviruses only infect dividing cells, lentiviral delivery system was more popular so that both dividing and non-dividing cells could be infected in the hopes of improving reprogramming efficiency.
Lentiviral plus cre-lox mediated transgene excision: To date, the first production of transgene free iPSCs were performed with lentiviral vectors containing loxP sites in the 5′and 3′LTR of the viral vectors. The existence of loxP sites gave a substrate to remove most of the transgene sequences by Cre-mediated recombination.
Adenovirus: Adenovirus is a non-integrating virus that does not infect replicating cells. The first known research reporting the production of iPSCs with an adenoviral vector was reprogrammed mouse tail tip fibroblasts to iPSCs. However, the reprogramming efficiency was in the range of 0.001–0.0001%, because the expression window of reprogramming factors is too narrow to induce the expression of endogenous factors required to transition somatic cells.

A minicircle vector composed of Lin28, GFP, Nanog, Sox2, and Oct4, and enables to be used to reprogram human adipose stem cells. Usually, the reprogram steps contain three transfections of the minicircle vector: an initial electroporation, followed by sorting of GFP+ cells, then two lipid-based transfections. With professional scientists devoted themselves in iPSC reprogramming, Creative Biolabs is dedicated to providing the first class iPSC reprogramming services for our customers. Our services will contribute greatly to the success of your iPSC programs. Please contact us for more information and a detailed quote.

Creative Biolabs is dedicated to offering the iPSC research and application with the highest-quality karyotyping service. With years of experience, our professional scientists can provide high-efficient iPSC karyotype analysis services for our customers all over the world.

Neuronal Activity Monitor

CNS drug discovery has been hampered by inadequate understanding or consideration of a number of factors. These include the complexity of brain anatomy and function; the neuro-PK with regard to the blood-brain barrier (BBB) transport and intra-brain distribution as well as the measures to study these processes; adequate biomarkers of CNS drug effects (neuro-PD), and the complex nature of CNS diseases. Also, the reductionist view and thereby lack of understanding of the interaction and interdependencies of all these factors have contributed to the high attrition rates of CNS drugs.

Neuroscience, also known as neurobiology, is the scientific study of the structure and function of the nervous system. Combined with multiple disciplines, it focuses on the study of the nervous system with different approaches and different scales. Neural tissue damage in the peripheral nervous system (PNS) and central nervous system (CNS) may result in multiple neurological diseases and even life-long disabilities. Neural tissue engineering is a promising strategy for neural repair and neurological diseases treatment. In this case, proper preclinical models are required to mimic the micro-environment. Here, Creative Biolabs has developed the STEMOD™ neuroscience ex vivo models for our clients all over the world.

Neuronal Differentiation Services

Neurotransmitters are the key players in transmitting signals between neurons, thus influencing a wide array of physiological and psychological processes. Understanding their levels, dynamics, and interactions is paramount for elucidating the underpinnings of conditions such as Parkinson's disease, Alzheimer's disease, depression, and anxiety disorders. Creative Biolabs provides a full range of in vitro neurotransmitter detection assays that enable researchers to comprehensively study different neurotransmitters.

Creative Biolabs' NeuSenso™ platform provides an integrated product ecosystem designed to simplify your research and production experience. We offer a portfolio of Sensory neuron products covering a wide range of use cases. Whether you want to study early or mid-stage developmental events, or are interested in the type of terminal cells that are produced, the NeuSenso™ platform can provide products that meet your specific needs.